library construction and sequencing Search Results


90
National Institute of Standards and Technology standard reference material 2374
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Complete Genomics Inc mgisp 100 system
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Average 95 stars, based on 1 article reviews
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Oxford Nanopore fragments 10 kbp and a library was constructed using a ligation sequencing kit
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Integragen sa sequence variant detection and annotation
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UCL Genomics library preparation and sequencing
Library Preparation And Sequencing, supplied by UCL Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/library+construction+and+sequencing/library+preparation+and+sequencing/pmc10176342__ard___2022___223469supp006-32-9-13
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SeqMatic LLC library preparation and sequencing
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https://www.bioz.com/product/library+construction+and+sequencing/library+preparation+and+sequencing/pm33086075-194-3-7
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LGC Genomics GmbH rna extractions, cdna synthesis library preparation and sequencing
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Vertis Biotechnologie library preparation and paired-end sequencing
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Average 90 stars, based on 1 article reviews
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GenScript corporation a plasmid construct consisting of approximately 500 bp of homologous sequence up and downstream of phop with an internal kanamycin cassette
Distribution and sequence coverage of Tn5 mutants in the S. praecaptivus genome are depicted as a histogram (A, Upper). Note that mutations affecting Sant_4061 are the most abundant in the library, following growth in LB media. The zoomed in regions of the histogram (A, Lower) reveal the distribution and abundance of Tn5 mutants in the genomic regions encoding phoPQ and Sant_4061 (in red), with the mutants recovered in the screen for polymyxin B sensitivity highlighted in orange, with the number of recovered mutants shown above each column. Growth curves for WT S. praecaptivus and Sant_4061 <t>and</t> <t>phoP</t> mutants grown in either LB or LB + Polymyxin B (PB) for 10 hours (B). Standard errors are shown above and below the average of three replicates at each time point. ΔSant_4061, ΔphoQ, and ΔphoP are unable to grow on LB supplemented with polymyxin B (C).
A Plasmid Construct Consisting Of Approximately 500 Bp Of Homologous Sequence Up And Downstream Of Phop With An Internal Kanamycin Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/library+construction+and+sequencing/a+plasmid+construct+consisting+of+approximately+500+bp+of+homologous+sequence+up+and+downstream+of+phop+with+an+internal+kanamycin+cassette/pmc05344769-328-4-30
Average 90 stars, based on 1 article reviews
a plasmid construct consisting of approximately 500 bp of homologous sequence up and downstream of phop with an internal kanamycin cassette - by Bioz Stars, 2026-10
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90
LGC Genomics GmbH gbs library preparation, sequencing and subsequent bioinformatic analysis
Distribution and sequence coverage of Tn5 mutants in the S. praecaptivus genome are depicted as a histogram (A, Upper). Note that mutations affecting Sant_4061 are the most abundant in the library, following growth in LB media. The zoomed in regions of the histogram (A, Lower) reveal the distribution and abundance of Tn5 mutants in the genomic regions encoding phoPQ and Sant_4061 (in red), with the mutants recovered in the screen for polymyxin B sensitivity highlighted in orange, with the number of recovered mutants shown above each column. Growth curves for WT S. praecaptivus and Sant_4061 <t>and</t> <t>phoP</t> mutants grown in either LB or LB + Polymyxin B (PB) for 10 hours (B). Standard errors are shown above and below the average of three replicates at each time point. ΔSant_4061, ΔphoQ, and ΔphoP are unable to grow on LB supplemented with polymyxin B (C).
Gbs Library Preparation, Sequencing And Subsequent Bioinformatic Analysis, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/library+construction+and+sequencing/gbs+library+preparation++sequencing+and+subsequent+bioinformatic+analysis/pmc06421318-134-13-5
Average 90 stars, based on 1 article reviews
gbs library preparation, sequencing and subsequent bioinformatic analysis - by Bioz Stars, 2026-10
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90
ATLAS Biolabs GmbH library preparation and sequencing
Distribution and sequence coverage of Tn5 mutants in the S. praecaptivus genome are depicted as a histogram (A, Upper). Note that mutations affecting Sant_4061 are the most abundant in the library, following growth in LB media. The zoomed in regions of the histogram (A, Lower) reveal the distribution and abundance of Tn5 mutants in the genomic regions encoding phoPQ and Sant_4061 (in red), with the mutants recovered in the screen for polymyxin B sensitivity highlighted in orange, with the number of recovered mutants shown above each column. Growth curves for WT S. praecaptivus and Sant_4061 <t>and</t> <t>phoP</t> mutants grown in either LB or LB + Polymyxin B (PB) for 10 hours (B). Standard errors are shown above and below the average of three replicates at each time point. ΔSant_4061, ΔphoQ, and ΔphoP are unable to grow on LB supplemented with polymyxin B (C).
Library Preparation And Sequencing, supplied by ATLAS Biolabs GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/library+construction+and+sequencing/library+preparation+and+sequencing/pmc07438112-408-3-7
Average 90 stars, based on 1 article reviews
library preparation and sequencing - by Bioz Stars, 2026-10
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Image Search Results


Distribution and sequence coverage of Tn5 mutants in the S. praecaptivus genome are depicted as a histogram (A, Upper). Note that mutations affecting Sant_4061 are the most abundant in the library, following growth in LB media. The zoomed in regions of the histogram (A, Lower) reveal the distribution and abundance of Tn5 mutants in the genomic regions encoding phoPQ and Sant_4061 (in red), with the mutants recovered in the screen for polymyxin B sensitivity highlighted in orange, with the number of recovered mutants shown above each column. Growth curves for WT S. praecaptivus and Sant_4061 and phoP mutants grown in either LB or LB + Polymyxin B (PB) for 10 hours (B). Standard errors are shown above and below the average of three replicates at each time point. ΔSant_4061, ΔphoQ, and ΔphoP are unable to grow on LB supplemented with polymyxin B (C).

Journal: Molecular microbiology

Article Title: The Regulation of Antimicrobial Peptide Resistance in the Transition to Insect Symbiosis

doi: 10.1111/mmi.13598

Figure Lengend Snippet: Distribution and sequence coverage of Tn5 mutants in the S. praecaptivus genome are depicted as a histogram (A, Upper). Note that mutations affecting Sant_4061 are the most abundant in the library, following growth in LB media. The zoomed in regions of the histogram (A, Lower) reveal the distribution and abundance of Tn5 mutants in the genomic regions encoding phoPQ and Sant_4061 (in red), with the mutants recovered in the screen for polymyxin B sensitivity highlighted in orange, with the number of recovered mutants shown above each column. Growth curves for WT S. praecaptivus and Sant_4061 and phoP mutants grown in either LB or LB + Polymyxin B (PB) for 10 hours (B). Standard errors are shown above and below the average of three replicates at each time point. ΔSant_4061, ΔphoQ, and ΔphoP are unable to grow on LB supplemented with polymyxin B (C).

Article Snippet: Construction of phoP and phoQ knockouts A plasmid construct consisting of approximately 500 bp of homologous sequence up and downstream of phoP with an internal kanamycin cassette was commercially produced (Genscript).

Techniques: Sequencing

Phylogeny of S. praecaptivus and related Sodalis-allied endosymbionts and free-living bacteria based on maximum likelihood analyses of the phoQ coding sequence (1.45 kbp) and 16S rRNA (1.46 kbp). Sequences below strain names on the phoQ phylogeny show the amino acids sequences of the Mg2+-binding site within PhoQ, with acidic residues highlighted in bold. The numbers adjacent to nodes indicate maximum likelihood bootstrap values shown for nodes with bootstrap support > 80%.

Journal: Molecular microbiology

Article Title: The Regulation of Antimicrobial Peptide Resistance in the Transition to Insect Symbiosis

doi: 10.1111/mmi.13598

Figure Lengend Snippet: Phylogeny of S. praecaptivus and related Sodalis-allied endosymbionts and free-living bacteria based on maximum likelihood analyses of the phoQ coding sequence (1.45 kbp) and 16S rRNA (1.46 kbp). Sequences below strain names on the phoQ phylogeny show the amino acids sequences of the Mg2+-binding site within PhoQ, with acidic residues highlighted in bold. The numbers adjacent to nodes indicate maximum likelihood bootstrap values shown for nodes with bootstrap support > 80%.

Article Snippet: Construction of phoP and phoQ knockouts A plasmid construct consisting of approximately 500 bp of homologous sequence up and downstream of phoP with an internal kanamycin cassette was commercially produced (Genscript).

Techniques: Bacteria, Sequencing, Binding Assay

Bacterial strains used in this study

Journal: Molecular microbiology

Article Title: The Regulation of Antimicrobial Peptide Resistance in the Transition to Insect Symbiosis

doi: 10.1111/mmi.13598

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: Construction of phoP and phoQ knockouts A plasmid construct consisting of approximately 500 bp of homologous sequence up and downstream of phoP with an internal kanamycin cassette was commercially produced (Genscript).

Techniques: